Narishige Pc10 Manual New Review

Turns the unit on; the "Heater Level" indicator should illuminate. Start Switch: Initiates the pulling process.

Pipette shape is determined by the balance of heat and weight: NARISHIGE WEB NEWS Heater Level:

Once the first pull is done, center the filament holder on the now-thinned part of the tube.

Remove the old coil and slide the leads of the replacement coil into the terminal blocks.

This is built like a tank. The mechanical solenoid is loud (a distinct CLACK sound), but it feels industrial and durable. The filament access is easy to open, making cleaning and filament replacement painless. The unit takes up a small footprint on the bench, which is a bonus for crowded electrophysiology rigs. narishige pc10 manual new

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Inspect the platinum coil daily. If it becomes warped, off-center, or coated with glass residue, it must be reshaped or replaced.

Keep the vertical slider tracks clean. Wipe them down with a lint-free cloth moistened with isopropyl alcohol. Do not use heavy oils, as they attract dust and cause friction.

Rotate the bottom switch 90 degrees clockwise to clear the weight path. Start Switch again. The unit will heat at the NO. 2 HEATER level and pull the glass until it separates. NARISHIGE WEB NEWS Reference Settings Examples Application First Pull Distance Thin/Long Pipette 3 (2L, 1H) Firm/Stubby Tip 4 (2L, 2H) Safety & Maintenance Tips No.051 Setting Values in the PC-10 Puller (Revised) Turns the unit on; the "Heater Level" indicator

Make sure the weights are properly attached and free to fall. 4. Operating the PC-10: Step-by-Step

Secure the top clamp first, ensuring the capillary is perfectly vertical.

: Clean blood or chemical stains with organic solvents like alcohol; do not boil for sterilization. Disassembly

To keep your new Narishige PC-10 operating at peak performance for years, follow a strict maintenance schedule. Calibration and Coil Care Remove the old coil and slide the leads

A key feature that set the PC-10 apart was its two pulling modes: a single-pull and a double-pull. The single-pull mode pulls the glass in one continuous stretch, ideal for longer, thinner needles. The double-pull mode, however, is where the PC-10’s versatility shines. It allows the heating and pulling parameters to be changed mid-process, enabling researchers to produce both long, thin needles for microinjection and robust microelectrodes for patch clamping on the same device. To adjust the pulling tension, the unit comes with four weights (two light and two heavy) that can be attached or removed to fine-tune the gravitational force.

Operating the Narishige PC-10 requires precise execution to protect the heating elements and ensure uniformity across samples. Step 4.1: Preparing and Loading the Glass

In the world of electrophysiology,显微注射 (microinjection), and patch-clamp techniques, the vertical micropipette puller is legendary. For decades, this robust, gravity-based puller was a staple in neurobiology and developmental biology labs worldwide. Despite newer digital models flooding the market, thousands of PC-10 units are still in active use—humming away on lab benches from Tokyo to Boston.

If the glass does not melt or separate, increase the heater dial by 2–3 units.