The Eppendorf 5402 is a legacy refrigerated microcentrifuge known for its durability and precise temperature control in molecular biology and clinical research labs. While it has been succeeded by newer models like the 5420 or 5424R, many 5402 units remain in active service.

Sets the duration of the run. Turning the knob fully counter-clockwise or past 99 minutes engages the continuous "Hold" mode.

When spinning infectious, toxic, or pathogenic samples, always utilize approved biosafety rotors or buckets with sealed caps. 3. Standard Operating Procedures (SOP)

Do not expose plastic rotor components or adapters to prolonged UV sterilization, as it weakens the structural integrity of the plastic. 5. Troubleshooting and Error Codes

The manual mandates the use of specific rotors. Using non-certified rotors voids the safety certification.

-4°C to +40°C, managed via digital controls .

Keep the lid open when the centrifuge is not in use to prevent mold and condensation build-up. Monthly Maintenance

Always secure the rotor lid before starting the run, particularly when using hazardous samples or aerosol-tight rotors. 2.3 Running the Centrifuge

The Eppendorf Centrifuge 5402 is a classic, refrigerated benchtop laboratory workhorse. Known for its reliability in spinning down microtubes, this model remains a staple in molecular biology, biochemistry, and clinical research labs worldwide.

Ensures the lid cannot be opened while the rotor is in motion. 2. Updated Operating Procedures

Shows the actual run speed. Note that older physical manuals primarily focus on RPM, but standard conversion charts allow you to calculate the exact G-force (RCF) based on the rotor radius. Temperature Management

Ensure no debris is blocking the lid pins. Press down firmly on the lid while pressing OPEN. Severe sample asymmetry or broken tube.