Following these guidelines helps you maintain high-quality results while optimizing resources. Next Step:
If you need a focused summary (e.g., QC ranges, step-by-step protocol, or differences from the prior edition), specify which section and I’ll generate a concise extract.
Check every shipment for cracked plates, frozen agar, excessive moisture/condensation, hemolysis in blood plates, discoloration, or signs of contamination. clsi m22a3 pdf
Chocolate Agar (except when used for fastidious organisms like Neisseria or Haemophilus ) Eosin Methylene Blue (EMB) Agar 2. Non-Exempt Media (Requiring Full User QC)
The standard explicitly applies to , ready-to-use microbiological culture media. It does not cover media prepared entirely in-house from raw ingredients (though those labs may still use it as a reference). Intended users include clinical labs, public health labs, veterinary diagnostics, and industrial microbiology facilities. Chocolate Agar (except when used for fastidious organisms
Although it has been superseded by newer regulatory frameworks like the IQCP in the United States, the document remains a technically valid resource, especially for international laboratories and for those seeking to understand the historical context and scientific principles behind media QC.
A central concept of CLSI M22-A3 is the division of quality control obligations. Intended users include clinical labs, public health labs,
These media have a higher inherent failure rate or are critical for isolating highly fastidious organisms. Laboratories must perform full QC testing on every new lot number received. Examples include Campylobacter agar and certain specialized selective media. Impact on Laboratory Regulatory Compliance
Test for using negative control organisms that should be inhibited.